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The rest of the procedure and the calculation are earned out as before. The liberated gases aro adjusted to a known volume The pressure pi of this volume of gas m the chamber is read on a mercury manometer Then the gases are oithor expelled from the chamber or absorbed by suitable reagents and the pressure pi IS read at the some volume os before absorption The partial pressure P of the gas at a given volume equals pi -- ps mm Hg.

From this difference the gas volume at OXl and mm, Hg can be calculated. Corroctions for vapor pressure and capillary attraction can be omitted slnco they are the same for both readings, provided that the temponiture remains constant this is gcaerally the case during the short time mtorval of the determination The barometric presBuro will also remam constant dutmg this penod.

The outer rubber tubmg is filled mth mercury with the aid of a Kma. The bulb of the leveling appiaratus has a volume of ml and a connected through 1 meter of vacuum tubing boiled m vaseline m vacuum to a Y-shaped jomt with 2 stopcocks.

Tbo stopcock a at the upper end of the Y jomt Bcrves to let out any posaible air bubbles which might have entered the tube while fillmg it with mercury The Bide arm is connected through rubber tubmg to the levelmg bulb A stopcock with a 3 mm bore connects the vertical arm to the man omoter and the chamber through c screw-clamped rubber tubmg.

The entire apparatus with tlie open monomotor is mounted on a board This board is attached m vertical poeitlon to the edge of a table near a water tap A hole is cut m the board to permit easy rotation of the extraction chamber A water motor or any motor with rpm con be used for shaking The chamber is shaken by means of a metal rod connected to an occontno wheel of the motor on one Hide and a clamp on the water jacket on the other For cleaning the mercury is removed and the apparatus is filled with cleaning solution then washed thoroughly with water and dried with acetone Finally air la sucked through to remove all the acetone.

This can bo achieved by regulating the position of the levelmg bulb and by closing the stopcock b The stop- cock grease as descnbed on page 28 is used but the stopcocks should not be removed and greased too often- The stopcocks should bo cleaned by wipfag them mth clean Imtfreo towels.

If a leak a present, oir nil! To tho dilute solution drops of capryllo alcohol arc added and tho mixture a well shaken before uso Procedure Dran-ing of the blood separation of the plasma and Its saturation with all colar air ore performed os descnbed for the x olu metric analj'sis.

The apparatus. The plasma is saturated with air of 6. The pressure pi of the eKtmcted gases is read at a volume of 0.

After food intake the CX j value rises and the alkali reserve drops, beginning approximately one hour after the meal and la st ing for sev eral hours.

In cases of mcreased ventilation of the lung a decrease in alkali reserv e IB found e. By rawmg tho levcimg bulb the manometer reservoir is filled partly mth mercury to establish a high pressure m tho apparatus.

This blue-green solution turns deep brown when oxygen is added Preparation of the blood sample The blood a taken with a dry or parafinaled syringe and transferred to a test tube contanmig a few Fla H Apparatos for Lceptof mceota free from gas.

The difference in mm Hg multiplied by the factor indicated m table results m the oxviren content m volume per cent. We fotmd that it IS not necessary to apply a correction factor as no absorption liquid IS mtroduced mto the chamber The CO content is obtomed by subtracting 1.

The stopcock of the pipet is closed. If no mercury pump is available the blood gas apparatus can be used as follows The pipet IS connected with rubber tubmg to the glass tube above stopcock a and with stopcock b closed the levelmg bulb is lowered, drawing mercury from a reservoir mto the pipet.

After mixmg the blood gases are liberated by ahakmg for three minutes and the pressure ts read at a volume of 0. By taismg the levelmg bulb the liquid level is brought to the height of the stopcock capillary to expel all air from the chamber During this process no blood must enter the stopcock capillary, sinoe the octyl alcohol, floating on the surface of the blood prevents the blood from leavmg the chamber Then 0 13 ml of feme cyanide solution plus excess femeyamde to account for the stopcock bore is mtroduced Into the apparatus.

The volume of the bore is usually marked by an arrow on the filling funnel. After the stopcock has been closed and sealed with mercury the chamber is evacuated by lou'enng the mar cury to the 60 mb maik and the chamber ls shakon under vacuum for three mmutoa.

The excess of salt solution is remoyed by suction with the stopcock open to the air The mercury is lowered to the 50 mb marie and the blood-salt solution mixture is saturated with air by shaking for three mmutes.

Then the oxygen is determined os described above. The blood gases are bberated by the addition U 0 07 ml of feme cyamdeHSaponin solution plus the volume of the stopcock bore the COj is absorbed with 0 1 ml of sodium hydroxide, and the oxygen with 0.

Procedure The hemoglobm is determined simultaneously according to Sahh and by detenmnmg the total oxygen capacity If the sample b weD standardized, one division of the hemometer scale corresponds to OlOgm of hemoglobm Otherwise a correction factor must be used.

Example The sample shows a total oxygen capacity of 19 09 voL per cent. This is also the case b states of blood dilution luch as occur m nephntis with hydremia.

In a normal person the circulatmg bemoglobm is satumted with oxygen m the lungs, so that b the arterial blood about 95 por cent of the amotmt of bemoglobb corresponding to the total capacity is saturated iMth oxygen Ihe arterial oxygen saturation can bo lowered by physical labor and drop below the normal range i e 92 per cent or leas.

In nervous persons with accelerated superficial respiration a lack of oxygen m the artenal blood has been observed, correspondmg to a saturation of 90 — 80 per cent and less.

In these persons no change of air occurs b the deeper respiratory passages the partial oxygen pressure b the alveolar air ts decreased and the hemogiobta is mcomplctely saturated with oxygen.

The same is fotmd m some decompensated heart diseases; carbon monoxide poisonbg and phosgene polsoamg are some of the toxic states that lead to decreased arterial oxygen saturation.

In moderate cases of phosgene polsonmg the bek of oxygen will not be severe dunng rest, but fa considcrnblo during physical work. The oxjgen saturation of venous blood vanes mdiflerent parte of the circulatory system and in tho antocubitol vem of a normal resting person it Is between 60 and 70 volumes per cent In patients with cyanotic heart diseases with circulatory disturbances the per centago of saturation in tho venous blood drops to 60 volume per cent or less.

Determination o? Durmg this time tho red cells wiD settle down and the sample must bo well mixed mth a glass rod before analysis.

Caro must be taken to avoid all contact with oir The oxygon content of this somplo is then determined The difference betvNCcn the oxygen content of tho last and tho first sample corre- sponds to the oxygen motaboham of the erythrocytes.

Bv:m-ple Oi content of the defibnnated blood dotennmed immediately O, content of the defibnnated blood after incubation 5.

VAK SLYK0 01 4 per cent, m anemia with active rejmerative processes losses up to 70 per cent may be found This method is advantageous because it provides a quantitative measurement of the regeneration and rt mdi cates any regenerative procoes even m caaee where it can not be demonstrated histologically The results are only valid under stnctlj sterilo conditions.

In our sample tho pH according to the figures is 7. Nkill J Biel Ciem 6! If 10 ml of siKer mtrato solution use up 9.

The solution is alloivcd to cool to room temperature and is titrated with potassium thiocyanate from a nucroburette till the appearance of on orange color For the blank determination 2 ml of solution I is used and treated like tho sample.

A nse of chlondea m serum occurs during anemia, m nephntis, essential hypertensian, cardiao decompensation, unne rstentioQ hyper?

One part leadb through rubber tubmg carrying a pbch clamp to Ri the other part m the same manner to Rt The entire apparatus is held in place by a clamp attached at Ri above the ground glass piece.

The glass tubings ipaclmg to and frcm the receiver are supported by rmgs. In such a case the solution is titrated with thiosulfate till the color disappears.

Now acid is added and the titraticm perfonnod aa usual The purified NaCI still contains traces of bromine, not more than of bromme per 6 Gm, of NaCl but smee only 0 Gm.

No hypochlonte has to be added to the receiver since the chlonde content of the sample and of the fuimel washings furnish a large excess of hypochlonte.

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In , after the 25 muddy acres chosen for the yards, shops and main terminal were drained and raised 3. The totally electrified railroad complex included: roundhouse, sand house, cinder pits, coal bin, four sets of movable coal chutes, turntable, oil house, machine shop, blacksmith shop, boiler shop, planing mill, cabinet shop, powerhouse, car repair shop, two water tanks, car scales, a store, an office, freight depot, 14 freight tracks and five repair tracks.

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3 Antworten

  1. Ich tue Abbitte, dass sich eingemischt hat... Ich hier vor kurzem. Aber mir ist dieses Thema sehr nah. Ich kann mit der Antwort helfen.

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